Journal: Neuron

Article Title: Cerebellar Control of Reach Kinematics for Endpoint Precision

doi: 10.1016/j.neuron.2019.05.007

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains AAV2-hSyn-hChR2(H134R)-mCherry UNC Vector Core N/A AAV2-hSyn-eArch3.0-EYFP UNC Vector Core N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory 000664 Mouse: Tg(Ntsr1-cre)GN220Gsat/ Mmucd Mutant Mouse Regional Resource Center 017266-UCD Gt(ROSA)26Sor tm32(CAG-COP4*H134R/EYFP)Hze The Jackson Laboratory 024109 Software and Algorithms MATLAB (versions: 2015b, 2018a) MathWorks https://www.mathworks.com/ Motive 1.9.0 Optitrack https://optitrack.com/products/motive/ Prism 7 GraphPad https://www.graphpad.com/ Arduino IDE 1.6.5 Arduino https://www.arduino.cc/en/main/software TestArduino T. Stavropoulos https://github.com/tstavropoulos/TestArduino Other 1.5 mm retroreflective markers B&L Engineering MKR-1.5 Food pellets: 20 mg, grain-based BioServ F0163 Camera lens, 10 mm focal length Edmund Optics 58204 Digital Power Supply Hewlett Packard E3630A Optical fiber, 0.125 mm ThorLabs FG105LCA Ceramic ferrule, 1.25 mm ThorLabs CFLC128 Laser, 470 nm Opto Engine LLC MDL-III-470–100mW Laser, 561 nm SLOC YL561T8–050FC Electrode interface board, 16 channel Neuralynx EIB-16 Digital headstage Blackrock Microsystems Cereplex M Data acquisition system Blackrock Microsystems Cereplex Direct Open in a separate window KEY RESOURCES TABLE Cerebellar anterior interposed nucleus (IntA) neurons are modulated near reach endpoint Closed-loop optogenetic manipulation of IntA adjusts reach kinematics in real time IntA activity is variably engaged to enhance reach endpoint precision Data provide a mechanistic insight into reach dysmetria

Techniques: Virus, Plasmid Preparation, Mutagenesis, Software